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Cy5 excitation spectra

WebMar 28, 2024 · Cy5 荧光染料是由北京索莱宝科技有限公司代理或销售的solarbio品牌的试剂，产品来源于北京市通州区联东U谷85A三层。北京索莱宝科技有限公司是中国最权威的Cy5 荧光染料试剂销售服务商之一，在北京等地方销售Cy5 荧光染料试剂已经多年。生物在线为您提供众多企业Cy5 荧光染料仪器产品及图片 ... WebAllophycocyanin (APC) Allophycocyanin (APC) is an intensely bright phycobiliprotein isolated from red algae that exhibits far-red fluorescence with high quantum yields. It is excited by laser lines at 594 and 633 nm, with an absorbance maximum at 650 nm and a fluorescence emission peak at 660 nm. APC is a large molecule that is chemically ...

Emission spectra of Cy3 and Cy5 DNA. - figshare

WebAll fluorochromes have excitation and emission spectra. The excitation spectrum is a range of light wavelengths that add energy to a fluorochrome, causing it to emit light in another range of wavelengths, the emission spectrum. ... (PE-Cy5 + PE overlap) - (PE overlap) = accurate PE-Cy5 results. General procedure. WebCy2 Cy3 Cy5 Spectra Viewer Use the spectra viewer below to compare Cyanine with other fluorophores, along with instrument specifics (laser/filter) for suitability in your assay. Read Our Blog About Selecting Fluorophores Using the Spectra Viewer Show Excitation and Emission Spectra Table 1. chad anema https://cervidology.com

Two-photon Excitation Spectra of Fluorochromes - Core Facility

WebA collection of spectra representing the microscope Hoffman Lab Olympus IX83 on FPbase. FP base. info. about FPbase help & FAQs how to cite FPbase contributing API recent activity how you can help contact. tools. ... Excitation Filters Semrock FF01-650/13 WebSpectrum [Cy5 (Cyanine-5)] Cy5 (Cyanine-5) is a fluorescent compound with an excitation peak at 651 nm and an emission peak at 670 nm. It can be excited using a 640 nm laser … WebThis Cyanine5 NHS ester (analog to Cy5 ® NHS ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires a small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reactions (please see our recommended protocol for more details). chad angeli

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Category:Cyanine Dyes (Cy2, Cy3, and Cy5) - Jackson ImmunoResearch

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Cy5 excitation spectra

Dimethylamino group modified polydopamine nanoparticles with …

WebApr 13, 2024 · A simple fluorescence resonance energy transfer (FRET) sensing platform (termed as USP), comprised of upconversion nanoparticles (UCNPs) as the energy donor and Cy5 as the energy acceptor, has been synthesized for cathepsin B (CTSB) activity detection in vitro and in vivo. When Cy5-modified peptide substrate (peptide-Cy5) of … WebCy5.5 is a far-red (and near-infrared) emitting dye which is ideal for fluorescence measurements where background fluorescence is a concern. It is also suitable for in vivo NIR imaging experiments. This reagent can …

Cy5 excitation spectra

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WebAll transmission and blocking (OD) data are actual, measured spectra of representative production lots. Spectra varies slightly from lot to lot. Optical density values in excess of … WebApr 22, 2014 · Titration of Cy5 tagged DNA (S2) into Cy3 tagged DNA (S1), showing resulting Cy5-Cy3 FRET upon duplex formation (excitation wavelength = 554 nm). The …

Web20 rows · Alexa Fluor dyes—Emission spectra. Figure 1. Emission … WebMar 28, 2024 · Cellular colocalization of Cy5-CpG 1826 and PDA NPs RAW264.7 cells were initially cultured in 6- well plate with the density of 15 × 10 4 cells per well. Then, 2 μL Cy5-CpG 1826 (Cy5-TCCATGACGTTCCTGACGTT , 1 μM, GenScript, China) was added into the cultured medium and incubated for another 4 h [ 39 , 40 ].

WebPerCP-Cyanine® 5.5 (PerCP-Cy5.5) is a red-emitting tandem fluorophore that combines PerCP and Cy5.5. The donor molecule, PerCP can be excited by the 488-nm blue laser and and transfers energy to the acceptor molecule, Cy5.5, which emitts light that can be captured with a 695/40 nm bandpass filter. WebMar 16, 2024 · In an emission spectrum, the excitation monochromator is set to some wavelength known to excite the sample and the emission monochromator is scanned …

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WebPE-Cy™5 Streptavidin. Fluorescence emission spectra of R-Phycoerythrin and PE-Cy5. Excitation is at 488 nm and the emission spectra are normalized to the absorbance of … chad and zWebPE (phycoerythrin)-Cy5.5 is a fluorescent compound with an excitation peak at 566 nm and an emission peak at 671 nm, giving it a fairly large Stokes' Shift of 105nm. It can be … chad and weWebAlexa Fluor® 488 (NHS Ester) 492. 517. ATTO™ 488 (NHS Ester) Alexa Fluor® 532 (NHS Ester) 527. 553. chad angelWebLaser line indicates the commonly used laser line (s) for detection using flow cytometry Common filter set indicates the standard microscope filter set that generates the best imaging results Excitation max indicates the peak excitation wavelength (see the Fluorescence SpectraViewer for a complete profile) chad angelocciWebSpectral overlap and FRET. The donor (Cy3) is excited at a maximal wavelength of 550 nm, and emits with light at 650 nm. The acceptor (Cy5) absorbs light at 570 nm and emits at 670 nm. Energy transfer occurs due to the spectral overlap between the emission of the donor and excitation spectrum of the acceptor (shown in green). Crosstalk occurs when … chad angeloWebFind compatible reagents by excitation source Each of the tabs below shows the antibody labels and application-based dyes and fluorescent reagents that are compatible with a given laser excitation source. Green laser configuration not available on the Attune CytPix model. Violet 6 (405 nm) Violet (405 nm) Blue (488 nm) Green (532 nm) chad anema city of menifeeWeb, k FCM RUO PerCP-Cy5.5 0.1 mg 551162 CD9 Hu M-L13 Ms IgG 1, k FCM RUO (GMP) PerCP-Cy5.5 50 tests 341649 CD11b (Integrin a M chain) Ms M1/70 Rat IgG 2b, k FCM RUO PerCP-Cy5.5 0.1 mg 550993 CD14 Hu M5E2 Ms IgG 2a, k FCM RUO PerCP-Cy5.5 0.1 mg 550787 CD16 Hu 3G8 Ms IgG 1, k FCM RUO (GMP) PerCP-Cy5.5 50 tests … hanover rust and dust days