Cst myc antibody

WebApr 11, 2024 · Anti-CK2α Antibody: Cell Signaling: 2656S: Anti-Puromycin (12D10) antibody: Millipore: MABE343: Secondary antibodies: Rabbit IgG HRP linked: GE Healthcare: NA934: Mouse IgG HRP linked: ... before immunoprecipitation with 4 μg of anti-Myc-tag antibody (Cell Signaling) per sample, preconjugated to protein G Dynabeads … WebIP. ChIP. Chromatin immunoprecipitations were performed with cross-linked chromatin from K-562 cells and either MTA3 (E3X2E) Rabbit mAb or Normal Rabbit IgG #2729, using SimpleChIP ® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR, using human Myc exon 2 primers and SimpleChIP ® …

Purified anti-c-Myc Antibody anti-c-Myc - 9E10 - BioLegend

WebHuman c-Myc is a helix-loop-helix transcription factor which efficiently binds DNA after heterodimerization with the bHLH protein Max. It is often overexpressed and mutated in hematopoietic tumors. Mutations frequently result in truncation around amino acid (aa) 252, before the C-terminal DNA binding, HLH and leucine zipper domains. Webc-Myc Antibody. Applications: WB, IHC, ICC/IF, IP, ELISA. Reactivity: Human, Mouse, Rat, Tag, Feline. Publications: 749. Images: 417. ... The c-Myc protein is a 62 kDa … fly out of australia https://cervidology.com

c-Myc Antibody from Cell Signaling Technology - Biocompare

WebACTL6B/BAF53B is a member of the neural-specific SWI/SNF complex that facilitates binding to target genes and is involved in memory and synaptic plasticity (13-15). ACTL6/BAF53 has been shown to interact with c-Myc, where it functions as a cofactor and is important in the transformation process (16). Further studies have shown … WebThis antibody detects c-myc protein and c-myc tagged proteins. MA1-980 has been successfully used in Western blot, immunohistochemistry, immunocytochemistry, immunofluorescence, ELISA, flow cytometry, and … WebUS11608381B2 US16/377,089 US202416377089A US11608381B2 US 11608381 B2 US11608381 B2 US 11608381B2 US 202416377089 A US202416377089 A US 202416377089A US 11608381 B2 US11608381 B2 fly out of xna

Myc-Tag (9B11) Mouse mAb from Cell Signaling Technology

Category:CST complex - Wikipedia

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Cst myc antibody

CST complex - Wikipedia

WebTarget Information. The c-Myc protein is a 62 kDa transcription factor that is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of … WebMouse monoclonal c-Myc antibody [8]. Validated in IHC and tested in Human. Cited in 6 publication(s). Independently reviewed in 6 review(s). Immunogen corresponding to recombinant full length protein.

Cst myc antibody

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WebAt CST, we adhere to the Hallmarks of Antibody Validation™, six complementary strategies for determining the specificity, sensitivity, and functionality of an antibody in any given assay. By carefully tailoring these strategies to each antibody product, we guarantee that CST antibodies will work as expected, to help you achieve results you ... WebThe proto-oncogene MYC plays a role in human oncogenesis. For more information see here.. This recombinant rabbit monoclonal antibody (Y69) to c-Myc specifically detects …

WebApr 13, 2024 · DNA fragments were incubated overnight with specific antibody c-Myc (E5Q6W, CST, USA) and control IgG. The next day, antibody-binding magnetic beads were added. Proteinase K was used to elute and digest the immunoprecipitated product. The purified DNA product was used for subsequent PCR detection (Table S2). Mouse IgG … WebICC staining of purified anti-c-Myc antibody (clone 9E10) on HeLa cells transfected with multi-tag plasmid containing c-Myc epitope sequence. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100, and blocked with 5% fetal bovine serum in PBS for 1 hour. The cells were then incubated with 1 µg/mL of the ...

Web6x-His Tag Antibody (MA1-21315) in ICC/IF. Immunofluorescent analysis of 6x-His Tag was performed using 70% confluent HEK-293 cells transfected with V5-H3-His construct. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature ... WebGet better batch-to-batch reproducibility with a recombinant antibody. Anti-c-Myc (phospho T58) antibody [EPR17923] (ab185655) Research with confidence – consistent and reproducible results with every batch. Long-term and scalable supply – powered by recombinant technology for fast production.

WebImmunofluorescent analysis of Transfected HEK-293 cells using 66004-1-Ig (MYC-tag antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This product is out of stock now, and we suggest you go to the replacement 60003-2-Ig. Our concentration is detected with BSA as standard.

WebAnti-Myc Tag, clone 4A6, is a mouse monoclonal antibody that is validated for use in ChIP, IC, IF, IP and WB for the detection of Myc Tag and does not show the very high context sensitivity of 9E10. This antibody has been cited in over 480 publications. Research Category. Epitope Tags & General Use. Research Sub Category. green pass clonatoWebThe mouse Anti-c-MYC monoclonal antibody recognizes an epitope located within a sequence (EQKLISEEDL) of the product of the human oncogene c-myc. c-Myc (v-myc avian myelocytomatosis viral oncogene homolog) is a transcription factor, which is coded by MYC gene. It was first described as a homolog of an avian retroviral oncogene. green pass claraWebThe c-Myc protein is a 62 kDa transcription factor that is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous ... flyout panelWebc-Myc (also known as Myc), together with l-Myc and n-Myc, belongs to the Myc family of transcription factors. c-Myc has a basic helix-loop-helix leucine zipper domain and through heterodimerization can bind and regulate the transcriptional activity of genes, either by repression or activation. It is a key player in the regulation of cell growth ... fly out of provo utahWebC – Pulmonary EC co-transfected with GFP-tagged CLIP-170, CLASP2, EB1 or β-tubulin and Myc-SOCS1 were tested in co-IP assay with GFP antibody followed by immunoblotting with Myc-antibody. Probing of total cell lysates with GFP and Myc antibodies was used to verify ectopic protein expression; membrane re-probing for β-actin was used to ... green pass come ottWebThe immunogen of 16286-1-AP is MYC tag Fusion Protein expressed in E. coli. PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC … green pass codice stpWebAnti-c-Myc , Cat. No. 06-340-I, is a rabbit polyclonal antibody that detects c-Myc and is tested for use in Immunocytochemistry and Western Blotting. Physical form Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide fly out to meaning